> User's Guide > Flux Balance Analysis > Optimization
FBA Objective Function Analysis

This process is used to compute the optimal values of a list of objective functions. The following steps are executed during this process:

1. The next objective function contained in the specified objective function file is retrieved, optimized in the specified direction, and the results are written to the next line of the specified output file.
2. If another objective function is present in the objective function file, Step 1 is repeated.

Here is the set of keywords this process understands, along with a description of their possible corresponding values.


Required Keywords Possible Values
Process FBA Objective Function Analysis
Reaction File The name of an FBA Reaction File containing a stoichiometric network.
Constraints File The name of a text file containing the user-defined flux constraints. See Constraints Files for further information.
Objective Function File The name of a text file containing the objective functions to be optimized. See Objective Function Files for further information.
Optimization Sense The sense, or direction, in which the optimal values will be computed. See Optimization Senses for further information.
Program Solver The name of the program solver to be used to compute the optimal values. See Program Solvers for further information.
Output File Name The name of the file to which the computed values will be written. See FBA Multiple-Optimization Output Files for further information.
Data Headers The data headers of the specified output file. See FBA Optimization Data Headers for further information.
Optional Keywords Possible Values
Constraint Tolerance The amount by which the linear program solver is allowed to violate the defined flux constraints. See Constraint Tolerances for further information.
Safety Level The safety level at which the optimization will be performed. See Safety Levels for further information.
Program Solver Parameter File The name of the file containing parameters for the linear program solver. See Program Solver Parameter Files for further information.
Objective Function File Format The format of the objective function file. See Objective Function Files for further information.

Examples

Click here for an example.